Investigation of cytokine gene polymorphisms in patients with psoriasis vulgaris

Psoriasis is associated with cutaneous and systemic overexpression of several proinflammatory cytokines. The aim of this study was to investigate the relationship between susceptibility to psoriasis and polymorphisms of tumor necrosis factor alpha (TNF-α), interferon gamma (INF-γ), interleukin (IL-10), IL-6 and transforming growth factor beta (TGF-β). Eighty-nine patients with psoriasis and 201 healthy controls were enrolled into the study. The patient group was divided into 2 subgroups as early-onset (group 1) and late-onset (group 2). The cytokine gene polymorphisms in each group were determined by polymerase chain reaction-single specific primer. When the whole and only group 1 patients were compared with the controls, TGF-β TT/GC genotype was significantly high in the whole and group 1 patients. When we compared the group 1 and group 2, the frequency of IFN-γ AA genotype was found to be significantly high in group 1 which lost significance after Bonferroni correction. Patients with moderate symptoms had a significantly high frequency of IL-10 GCC/GCC genotype that did not remain significant after correction. These data from our small group of patients demonstrated that the only significant difference between the whole patient group and the controls was for TGF-β TT/GC genotype with a higher frequency in the patients. Due to the involvement of many other genes relating to the activity of Th1 cytokines, further studies are required to determine the molecular basis of the susceptibility to psoriasis.

Psoriasis vulgarisli hastalarda sitokin gen polimorfizminin araþtýrýlmasý

Psoriasisde enflamasyondan sorumlu Th hücrelerin ürettikleri proenflamatuvar sitokinler hastalýðýn patogenezinde önemli yer tutarlar. Bu çalýþmanýn amacý, psoriasis hastalýðý ile patogenezde rol alan tümör nekrozu faktörü alfa (TNF-α), interlökin (ÝL-10), interferon (ÝFN-γ), ÝL-6, dönüþtürücü büyüme faktörü beta (DBF-β) gen polimorfizmleri arasýndaki olasý iliþkiyi saptamaktýr. Çalýþmaya psoriasis tanýsý konmuþ 89 hasta ile 201 saðlýklý birey dahil edilmiþtir. Hasta grubu, erken baþlangýçlý (grup 1) ve geç baþlangýçlý (grup 2) olarak ikiye ayrýlmýþtýr. Ýki grubun sitokin gen polimorfizmleri polimeraz zincir reaksiyonu-sekans spesifik primer yöntemi ile ticari kit kullanýlarak tiplenmiþtir. Tüm hastalar ile grup 1 hastalarýný ayrý ayrý kontrol grubu ile karþýlaþtýrdýðýmýzda DBF-β TT/GC haplotipi hastalarda anlamlý olarak yüksek bulundu (p<0,05). Grup 1 ve grup 2 karþýlaþtýrýldýðýnda ÝFN-γ AA genotipi grup 1 hastalarda anlamlý olarak yüksek deðerlendirildi (p<0,05). Bonferroni testi sonrasý bu anlamlýlýk gözlenemedi. Orta þiddetli semptom gösteren bireylerde anlamlý olarak yüksek bulunan ÝL-10 GCC/GCC genotipi doðrulama sonucu anlamlýlýðýný kaybetti. Bu veriler, tüm hasta ve kontrol grubundaki tek farkýn DBF-β TT/GC genotipinin hastalarda anlamlý olarak yüksek olduðunu gösterdi. Th1 sitokinlerinin aktivitesi ile iliþkili diðer birçok genin varlýðý nedeniyle psoriasisin moleküler temelini belirlemek için yeni çalýþmalar gereklidir.