Glutatyon S-transferaz gen polimorfizmleri ile psoriazisin klinik özellikleri aras?ndaki ili?ki: Türk popülasyonunda olgu kontrol çal??mas?

Amaç: Bu çal??mada Glutatyon S-transferaz Mu 1 (GSTM1) ve Glutatyon S-Transferaz Pi 1 (GSTP1) gen polimorfizmlerinin psoriazis geli?imi ve özellikleri aç?s?ndan risk faktörü olup olmad???n? ara?t?rd?k.
Gereç ve Yöntem: Hasta ve kontrollerden 2 mL’lik EDTA’l? tüplere kan örnekleri al?nd?. ?ki yüz altm?? psoriazis hastas? ve 200 sa?l?kl? kontrolden DNA izolasyonu yap?ld? ve GSTM1 ve GSTP1 gen polimorfizmleri gerçek zamanl? polimeraz zincir reaksiyonu yöntemiyle genotiplendirildi.
Bulgular: Analiz sonuçlar?na göre hastalarda GSTM1 null genotip s?kl??? kontrollere göre 1,33 kat daha yüksek olmas?na ra?men bu fark istatistiksel olarak anlaml? de?ildi [%95 güven aral??? (GA): 0,91-1,93, p=0,13]. GSTP1 AG heterozigot genotip ve GG homozigot polimorfik genotip frekanslar? da hastalar ve kontroller aras?nda farkl?l?k göstermedi (AG genotipi için [olas?l?k oran? (OO): 0,80, %95 GA: 0,55-1,18, p=0,27; OO: 0,74, %95 GA: 0,35-1,56, GG genotipi için p=0,42]. Her iki polimorfizm hastal???n ba?lang?ç ya?? ve ?iddetine göre de?erlendirildi?inde erken ba?lang?çl? ya? grubu ile geç ba?lang?çl? ya? grubu aras?nda ve hafif grup ile a??r grup aras?nda anlaml? fark bulunamad?.
Sonuç: Sonuçlar GSTM1 ve GSTP1 polimorfizmlerinin psoriazisin etiyopatogenezi ve klinik özellikleri üzerinde önemli bir etkisinin olmad???n? göstermektedir.

Anahtar Kelimeler: Psoriasis, GST, gen polimorfizmi, hücresel detoksifikasyon

Relationship between Glutathione S-transferase gene polymorphisms and clinical features of psoriasis: A case-control study in the Turkish population

Background and Design: In this study, we investigated whether Glutathione S-transferase Mu 1 (GSTM1) and Glutathione S-transferase Pi 1 (GSTP1) gene polymorphisms are risk factors for psoriasis development and characteristics of psoriasis.
Materials and Methods: Venous blood samples were collected from both the patients and control subjects into 2 mL EDTA tubes. DNA was isolated from 260 psoriasis patients and 200 healthy control subjects, and GSTM1 and GSTP1 gene polymorphisms were genotyped by realtime polymerase chain reaction method.
Results: According to the analysis results, although the frequency of the GSTM1 null genotype was 1.33 times higher in patients compared to controls, this difference was statistically insignificant [95% confidence interval (CI): 0.91-1.93, p=0.13]. GSTP1 AG heterozygous genotype and GG homozygous polymorphic genotype frequencies also did not differ between patients and controls [odds ratio (OR): 0.80, 95% CI: 0.55-1.18, p=0.27 for AG genotype; OR: 0.74, 95% CI: 0.35-1.56, p=0.42 for GG genotype]. When both polymorphisms were evaluated based on the onset age and severity of the disease, no significant difference was found between the early onset age group and the late onset age group, nor between the mild group and the severe group.
Conclusion: The results show that GSTM1 and GSTP1 polymorphisms do not have a major effect on the etiopathogenesis and clinical characteristics of psoriasis.

Keywords: Psoriasis, GST, gene polymorphisms, cellular detoxification